And if you're a gamer, you've no doubt heard of the battles and surprise attacks that costs thousands of real-world dollars and that took weeks, if not, months of planning. The universe of EVE Online lives and breathes based on what its loyal and dedicated community decides to do. Compared with the growth of the reference strain (WT), which ha d a ma xi mumspec iﬁ c gr ow th ra te of 0.From being able to manipulate the in-game markets and the economy through mining resources, stealing them, or interfering with mining operations, to having spies work undercover in opposing alliances for years, just to betray them once they gain command of key assets. Taken together, these re- sults proved that we have successfully constructed a hybrid- gly co lys is ye ast, gra fti ng the EMP pa thw ay wit h the PK pa thw ay. This result indicated that TH enables pulling more ﬂux through the oxPP pathway. The decreased ratio of M+1 labeling of R5P/X5P/RL5P also proved that the carbon ﬂux into the oxPP in the QL5 strain was higher than that in the WT. The resul ts show ed that both in the WT or the QL5 stra in, the R5 P/X5P /RL5P we re main ly unla bele d (M+0)( Figu re 1 C),indic ati ng tha t in bot h str ai ns theﬂux goi ng int o thePP pat hwa y was mai nly viathe oxP P pat hwa y. To test how much carbon ﬂux went into the non-oxidative PP pathway via fructose-6-phosphate (F6P) in the QL5 strain, the M+1 labeling of R5P/X5P/R L5P was also test ed. The labeling experi- ments show that we successfully realized the pathway rewiring fro m the EMP pa thw ay to the oxP P pat hwa y.

Thi s was fur the r co nﬁr med by the hig her rat io o f M+ 0 glu ta - mate, which was derived from acetyl-CoA. Thi s res ul t ind ica tedthat thecarb on ﬂux of theEMP pat hwa y was successfully disrupte d and rewired in the hybrid-glycolysis yeast QL5. 50% labeling of both M+0 and M+1 in phosphoenolpyruvate (PEP), 2/3- phos phog lyce rate (2PG/ 3PG), and ala nine deriv ed from pyruvate generation, whereas there was almost no M+1 la- bel ingin PEP, 2PG /3P G, andalan inein theQL5 str ai n ( Figu re 1 C).

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